LABORATORY INSTRUMENTS



Centrifuge

Centrifuge is used to sediment or deposit rapidly particles
such as cells which may be suspended in a fluid. The speed
is expressed as rpm, i.e. revolutions per minute.

Relative Centrifugal Force (RCF)

More important than rpm is relative centrifugal force
(RCF). RCF is expressed as the acceleration due to gravity
or G (dynes per cm). The formula is:
G = 0.00001118 × (r) × (n)2
where r = radius in centimeters
and n = revolutions per minute.
The time of centrifugation is equally important. The tubes
should be spun for a definite period to obtain the desired
effect.

Types of Centrifuge

Hand Centrifuge

Fixed to the bench, the handle is rotated manually. It gives
low speeds only.

Motor-driven Centrifuge
Operated through mains electricity supply. The tubes may be kept in a fixed angle head or in a swing out head
Motor driven centrifuge with rpm. 
Microhematocrit Centrifuge

Also motor driven for finding out packed cell volume (PCV) of red blood cells (RBCs). In this, blood-filled capillary tubes are spun and later the percentage of RBC-filled column is estimated


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